A Novel Method for the Quantitative Detection of Sericin Content in Silk Fiber Based on the Ratio of Aspartate to Alanine

The development of a method for the quantitative determination sericin content ratio (SCR) is urgently needed silk refining and purification fibroin biomedical applications. In this work, series sericin/fibroin mixed samples with known SCRs were prepared by mixing initial extracted from Bombyx mori silk. Significant differences found in contents characteristic hydrophilic amino acids abundant hydrophobic fibroin, several linear relationships SCR associated ratios Ser/Ala, Asp/Ala, Lys/Ala, Asp/Gly Ser/Gly established acid analysis. Subsequently, equation expressing (%) as function Asp to Ala X was = 2.5634X − 12.5587 (R2= 0.9972). results indicated that degummed silks calculated more objective effective than obtained traditional weight loss method. Our study provides novel approach sensitive detection within limit unknown silks, which can contribute quality control production process.